CD205 Monoclonal Antibody (205yekta), NovaFluor™ Red 685, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells. Applications Tested: This 205yekta antibody has been tested by flow cytometric analyis of mouse bone marrow cells. This can be used at less than or equal to 0.2 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Use 5uL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser