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CD14 (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Código de producto. 17815723
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Cantidad:
2 x 500 μg
25 μg
50 μg
500 μg
Tamaño de la unidad:
1 miligramo
25 microgramos
50 microgramos
500 microgramos
Código de producto. Cantidad unitSize
17815723 2 x 500 μg 1 miligramo
17834873 25 μg 25 microgramos
17895083 50 μg 50 microgramos
30054541 500 μg 500 microgramos
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Código de producto. 17815723

Marca: Invitrogen 500P320BT1MG

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Descripción

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human CD14-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD14 (500-P320) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sCD14. Western Blot: To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD14 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P320BT-1MG will be provided as 2 x 500 μg

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno CD14 (soluble)
Aplicaciones ELISA, Western Blot
Clasificación Polyclonal
Concentración 0.1-1.0 mg/mL
Conjugado Biotin
Formulación PBS with no preservative
génica Cd14
N.º de referencia del gen P08571
Alias de gen CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
Símbolos de los genes Cd14
Especie del huésped Rabbit
Inmunógeno HEK293 cells-derived Recombinant Human sCD14
Método de purificación Antigen affinity chromatography
Cantidad 2 x 500 μg
Estado normativo RUO
Primario o secundario Primary
ID de gen (Entrez) 929
Especies diana Human
Contenido y almacenamiento -20°C
Formulario Lyophilized
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