PeproTech beta-Defensin 1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Descripción

AA Sequence of recombinant protein: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-1. Anti-Human BD-1-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hBD-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-1 (500-P253) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-1. Western Blot: To detect hBD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P253BT-1MG will be provided as 2 x 500 μg

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis.