Invitrogen™ Kit de ELISA competitivo para péptido natriurético auricular (ANP)

Descripción

The Atrial Natriuretic Peptide (ANP) Competitive ELISA quantitates ANP in human plasma,urine or cell culture medium.

Principle of the method

The Atrial Natriuretic Peptide (ANP) Competitive ELISA research-use-only kit is designed to quantitatively measure ANP present in plasma,urine,and tissue culture media samples. This kit is not recommended for serum or plasma samples without extraction. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

La ANP se deriva de la proteína larga del aminoácido 151 preproANP3. La proteína prepro se escinde para generar proANP. ANP se deriva de aminoácidos 99-126 para formar el péptido de aminoácidos 28 con un enlace de disulfuro entre los aminoácidos 7 y 23. La ANP es el miembro predominante de una familia de hormonas peptídicas relacionadas estructural y funcionalmente que ejercen una amplia gama de efectos sobre la función cardiovascular y renal. La ANP es idéntica en un gran número de especies. Las ANP humana, de cerdo, oveja y perro tienen secuencias idénticas y este kit medirá muestras de estas especies de manera idéntica. Las moléculas de ANP de rata y ratón difieren en el aminoácido 12 pero muestran una reactividad cruzada completa con este kit.