Invitrogen™ Apolipoprotein C2 Human ELISA Kit

Descripción

The Human Apolipoprotein C2 (Hu ApoC2) ELISA quantitates Hu ApoC2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoC2. Principle of the method The Human ApoC2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Las apolipoproteínas son componentes proteicos de las lipoproteínas plasmáticas. La familia de genes de la apolipoproteína C codifica cuatro proteínas homólogas designadas apoC-I a apoC-IV, que modulan específicamente el metabolismo de las lipoproteínas ricas en triglicéridos. El gen apoC-I humano se asigna al cromosoma 19q13.2 y se expresa principalmente en el hígado, donde se activa cuando los monocitos se diferencian en macrófagos. El gen apoC-II humano se asigna al cromosoma 19q13.2 y codifica una proteína de cadena única de 79 aminoácidos que es un cofactor necesario para la activación de la lipoproteína lipasa, la enzima que hidroliza los triglicéridos en el plasma y transfiere los ácidos grasos a los tejidos.