p16(INK4) Mouse anti-Human, Unlabeled, Clone: G175-1239, BD

Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. The p16[INK4] protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. p16[INK4] inhibits cdk4 dependent phosphorylation of the tumor suppressor retinoblastoma protein (Rb) and Rb related proteins, p107 and p130. The biochemical properties of p16[INK4] suggest that it may be a tumor suppressor gene product. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MST1) has been identified as the gene for p16[INK4]. The gene, now also known as the CDKN2 gene, has been found to be mutated in a very high percentage of tumors, including 75% of melanoma cell lines. p16 migrates at a reduced molecular weight of 16kDa. G175-1239 reacts with human p16. A full-length recombinant bacterially produced GSTp16[INK4] fusion protein was used as immunogen. The hybridomas were screened by ELISA, immunoprecipitation and western blot analysis. The supernatant was also tested against GST protein to rule out reactivity to this portion of the immunogen. Endogenous p16 has been detected in several cell lines including Saos-2 human osteosarcoma (ATCC HTB 85), WI-38 human lung fibroblast (ATCC CCL 75), HeLa cervical carcinoma (ATCC CCL 2), and 293 adenovirus immortalized human kidney (ATCC 1573) cells. These cell lines are suggested as positive controls. U-2 OS human osteosarcoma (ATCC HTB 96) and MCF7 human breast carcinoma (ATCC HTB 22) have been shown to have undetectable levels of p16 and are suggested as negative controls.

Host Species: Mouse
Clone: G175-1239
Isotype: IgG1
Species Reactivity: Human
Immunogen: GSTp16[INK4] recombinant fusion protein
Formula Weight [Chemical]: 16kDa

Immunoprecipitation, Western Blotting