CD274 Mouse anti-Human, Brilliant Violet 421, Clone: MIH1, BD

The MIH1 monoclonal antibody specifically binds to CD274, also known as Programmed Death Ligand 1 (PD-L1) and B7 Homolog 1 (B7-H1). CD274/PD-L1 and CD273/PD-L2 are members of the B7 family within the Immunoglobulin superfamily. These type I transmembrane glycoproteins function as ligands for CD279, likewise known as the Programmed Death 1 (PD-1) receptor. CD274 is expressed on activated dendritic cells, macrophages and T cells. Studies show some overlapping functions of CD274 and CD273 and indicate an important role for CD279 and its ligands in regulating T and B cell responses. CD274 is also expressed on placental trophoblasts, myocardial endothelium, cortical thymic epithelial cells, keratinocytes, and carcinomas. The MIH1 antibody is reportedly a blocking antibody.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.