Invitrogen™ Amyloid beta 40 Human ELISA Kit

Descripción

The Human Amyloid Beta 40 (Hu Abeta 40) ELISA quantitates Hu Abeta 40 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 40. Principle of the method The Human Abeta 40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Beta amiloide es un péptido de 40-43 aminoácidos escindido de la proteína precursora amiloide y una gamma-secretasa putativa. Beta amiloide (1-40) junto con beta amiloide (1-42) son dos variantes C-terminal principales de la proteína beta amiloide, que constituyen la mayoría de las proteínas beta amiloides. Las aloformas beta amiloide (40) y beta amiloide (42) son componentes principales de las placas extracelulares que se encuentran en el tejido cerebral con enfermedad de Alzheimer.

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