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Applied Biosystems™ Reactivos de RT-PCR de un paso AgPath-ID™ con manual
Tienda Applied Biosystems ProductosDescripción
- Recommended for RNA pathogen amplification
- Consistent amplification of RNA targets with high specificity and sensitivity
- Optimized to work with your target-specific primers and probes
- Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
Especificaciones
Especificaciones
| Concentración | 25X |
| Contenido y almacenamiento | Sufficient for 1000 reactions of 25 μL, contains: • 14 mL 2 x RT-PCR Buffer • 1100 μL 25 x RT-PCR Enzyme Mix • 25 mL Nuclease-free Water. |
| Descripción | Works on all AB platforms, but is optimized for the 7500. |
| Método de detección | Sonda de cebado |
| Formato | Tubo |
| GC-Rich PCR Performance | High |
| Método de PCR | 1-step RT-qPCR |
| Polimerasa | ADN polimerasa AmpliTaq Gold |
| Velocidad de reacción | Estándar |
| Para utilizar con (equipo) | Sistema 7500 |
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
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