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  6. TdB Labs AB 20 kDa FITC-dextran Green Fluorophore

TdB Labs AB 20 kDa FITC-dextran Green Fluorophore

Código de producto. 30216458
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Cantidad:
100 mg
1 g
5 g
Tamaño de la unidad:
1 g
100 miligramos
5 g
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Código de producto. 30216458

Marca: TdB Labs AB FD20_100mg

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Descripción

FITC-dextran 20 kDa is a green-fluorescent, neutral dextran widely used in permeability, diffusion, and transport studies. Its intermediate size makes it ideal for modeling selective barrier passage in cells and tissues.

  • Product Overview: FITC-dextrans are dextran molecules covalently labeled with fluorescein isothiocyanate (FITC), providing bright green fluorescence ideal for permeability studies, molecular tracking, and bioimaging applications. Available in a wide range of molecular weights from 4 kDa up to 2000 kDa, FITC-dextrans are widely used in studies of barrier integrity, vascular leakage, macromolecular diffusion, and cellular uptake in biological systems.
  • Features:
    • Bright Green Fluorescence: Excitation at 495 nm and emission at 519 nm, compatible with standard FITC filter sets for microscopy, plate readers, and flow cytometry.
    • Wide Molecular Weight Range: Available from 4 kDa to 2000 kDa to suit studies involving different diffusion rates and molecular size exclusion properties.
    • Water-Soluble and Biocompatible: Fully soluble in aqueous solutions, enabling direct use in cell culture, tissue studies, and in vivo models.
    • High Lot-to-Lot Consistency: Manufactured with controlled degree of labeling and tight molecular weight distribution for reproducibility across experiments.
    • Flexible Detection Methods: Suitable for both live imaging and endpoint assays involving fluorescence microscopy, spectrophotometry, or flow cytometry.
  • Benefits:
    • Quantitative Analysis of Barrier Function: Widely used to measure permeability across cellular monolayers, tissues, and engineered barriers.
    • Modeling Molecular Diffusion: Different molecular weights allow precise modeling of size-dependent diffusion across biological membranes and matrices.
    • Tracking Macromolecular Transport: Useful for studying vascular permeability, blood-brain barrier integrity, and nanoparticle delivery models.
    • Suitable for Live and Fixed Systems: Can be applied in live cell experiments as well as fixed tissue studies without significant loss of fluorescence.
    • Standardized Fluorescence Signal: FITC labeling ensures a strong, predictable fluorescence signal across different imaging platforms.
  • Applications:
    • Barrier Permeability Assays: Used to evaluate tight junction function and paracellular transport in epithelial and endothelial models.
    • Vascular Leakage Studies: Applied in models of inflammation, stroke, cancer, and injury to assess changes in vascular permeability.
    • Macromolecular Diffusion Research: Traces molecular movement in microfluidic devices, 3D cultures, and organoids.
    • Cellular Uptake and Endocytosis Studies: Used to monitor macropinocytosis, vesicular trafficking, and intracellular transport dynamics.
    • Preclinical Drug Delivery Studies: Models the behavior of therapeutic macromolecules in biological systems.
  • Technical Specifications:
    • Fluorescence: Excitation maximum at 495 nm, emission maximum at 519 nm.
    • Molecular Weights: Available at 4 kDa, 10 kDa, 20 kDa, 40 kDa, 70 kDa, 110 kDa, 150 kDa, 500 kDa, and 2000 kDa options.
    • Appearance: Supplied as a light-sensitive, greenish powder, readily soluble in water.
    • Degree of Labeling: Optimized for bright signal without altering dextran diffusion behavior significantly.
  • Storage and Stability:
    • Store dry, protected from light, at 2°C to 8°C for maximum stability.
    • Stable under recommended conditions for extended storage periods without significant degradation of fluorescence intensity.
  • Packaging and Availability:
    • Supplied as dry powder for easy handling and storage.
    • Available in a range of packaging sizes to accommodate small-scale experiments and large research projects.

Especificaciones

Cantidad 100 mg
Tipo de producto Fluorescent Labeling Reagent
Peso molecular 20 kDa
Tipo de etiqueta Other Labels or Dyes
Color Green
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