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  6. TdB Labs AB 500 kDa Eosin-Y-lysine-dextran Yellow-green Fluorophore

TdB Labs AB 500 kDa Eosin-Y-lysine-dextran Yellow-green Fluorophore

Código de producto. 30216314
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Cantidad:
10 mg
25 mg
Tamaño de la unidad:
10 miligramos
25 miligramos
Código de producto. Cantidad unitSize
30216314 10 mg 10 miligramos
30216459 25 mg 25 miligramos
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Código de producto. 30216314

Marca: TdB Labs AB EYLD500_10mg

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Beschreibung

Eosin Y-lysine-dextran 500 kDa is a high molecular weight, pH-sensitive fluorescent polymer used in permeability assays and live/dead staining. The lysine linker enables better fixation and conjugation for cell-based and imaging studies.

  • Product Overview: Eosin Y-lysine-dextran is a water-soluble, fluorescently labeled dextran designed for enhanced conjugation and fixation. By introducing lysine groups onto the dextran backbone, the product enables more efficient crosslinking to biomolecules and surfaces. It is ideal for permeability studies, intracellular tracking, and stable imaging applications requiring strong, visible-range fluorescence.
  • Features:
    • Visible Spectrum Fluorescence: Exhibits strong absorbance around 517 nm and emission around 540 nm, compatible with standard fluorescence microscopy setups.
    • Lysine Modification: Provides additional amine groups, facilitating conjugation to biomolecules, surfaces, or matrices and improving fixation in cells and tissues.
    • Water-Soluble: Retains excellent solubility due to the dextran backbone, allowing for simple preparation and integration into aqueous experimental systems.
    • Multiple Molecular Weights Available: Offered in various sizes to support different permeability, diffusion, or imaging needs.
    • High Lot-to-Lot Consistency: Carefully controlled production ensures reproducibility of fluorescence intensity, molecular weight, and substitution degree.
  • Benefits:
    • Enhanced Conjugation Capability: Lysine groups allow chemical coupling to proteins, antibodies, or surfaces, making it highly versatile for custom assay development.
    • Stronger Fixation: Improved retention during cell fixation and tissue processing compared to standard fluorescent dextrans without lysine groups.
    • Reliable Barrier and Transport Studies: Bright, stable fluorescence makes it ideal for assessing molecular movement across cell layers, blood vessels, and engineered tissues.
    • Flexible Experimental Design: Available in different molecular weights to allow selection based on desired diffusion rate and system complexity.
    • Visible Fluorescence Without Special Equipment: Easily detected with common FITC/TRITC filter sets without needing specialized optics.
  • Applications:
    • Permeability Assays: Used to evaluate barrier function across epithelial, endothelial, and engineered tissue layers.
    • Intracellular Transport Studies: Traces molecular movement inside cells, particularly during uptake and transport experiments.
    • Surface Conjugation: Enables stable attachment to biomaterials or experimental platforms through available amine groups.
    • Fixed and Live Imaging: Suitable for both live-cell tracing and post-fixation imaging workflows due to improved crosslinking.
    • Vascular Leak Models: Used in in vivo or ex vivo models to assess vascular permeability and tissue distribution of macromolecules.
  • Technical Specifications:
    • Fluorescence: Absorbance maximum 517 nm, emission maximum 540 nm.
    • Molecular Weights: Available in multiple sizes to match experimental system requirements.
    • Appearance: Supplied as a red to orange powder, readily soluble in water.
    • Degree of Substitution: Controlled within low ranges to preserve dextran solubility and biological compatibility while enabling conjugation.
  • Storage and Stability:
    • Store dry and protect from light in sealed containers at ambient temperature.
    • Stable for extended periods under recommended storage conditions, preserving fluorescence and reactivity.
  • Packaging and Availability:
    • Supplied as dry powder for ease of handling and extended shelf life.
    • Available in a range of packaging sizes suitable for both small-scale research and larger experimental workflows.

Spezifikation

Cantidad 10 mg
Tipo de producto Fluorescent Labeling Reagent
Peso molecular 500 kDa
Tipo de etiqueta Other Labels or Dyes
Color Green
Name des Produkts
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