Thermo Scientific U/HPLC columns and consumables

See Your Biotherapeutics in High Resolution

Thermo Scientific U/HPLC columns and consumables

Featured Products
Gene biotherapeutics
Protein therapeutics
Resources

As leaders of innovative, reliable HPLC and UHPLC column development for over 40 years, you can trust Thermo Scientific^TM columns to meet your needs for complex separations and analyses. Our columns for biomolecule separation – referred to as BioLC columns – were designed for high resolution, enhanced sensitivity, faster analyses, and consistent performance. Browse our recommended columns for your applications below.

AAV’s
Aggregates
Bioanalysis- large molecules
Charge variants
Drug discovery/development
Glycan analysis
Intact protein/subunit
Oligonucleotides
Peptide mapping

Click here to explore our complete Thermo Scientific Chromatography consumables offering.

Thermo Scientific™ Biopharma Workflow Enablers

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Find Your Next Bio-LC Column

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Biopharmaceutical Analysis (U)HPLC Learning Center

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Advances in genetics and bioengineering have enabled the development of
biotherapeutics with unprecedented potential to treat diseases caused by
recessive gene disorders, acquired genetic diseases, and some viral
infections. Technologies for manufacturing and analysis of gene, mRNA, and
oligonucleotide based biotherapeutics such as chromatographic methods
are evolving with continued efforts to be fully optimized.

High Resolution DNA/RNA Separation

Revolutionize your oligonucleotide analysis with game- changing DNAPac RP columns.

AAV- Gene Therapies

Maximize efficiency, minimize interactions with our innovative MDiTM tech. to optimize Aggregate Analysis of AAVS.

SEC HPLC columns for Aggregate Analysis

Monitor AAV aggregates and monomers
Size-exclusion chromatography (SEC)

SurePac Bio 550 SEC MDi

Oligonucleotide HPLC & UHPLC columns

Separate oligonucleotide and nucleic acid mixtures including siRNA and mRNA
Anion-exchange and reversed phase (RP) column chemistries

DNAPac RP DNAPa200(RS) DNASwift

Sample Handling SureSTART GOLD-grade

Hydrophobic proteins and peptides tend to create salt adduct swith glass vials over time, reducing the recovery of certain proteins or peptides.
Thermo Scientific™ SureSTART TM GOLD-grade vials have an ultra-low adsorption glass surface that enables trace-level analysis or strongly adsorbing analytes like proteins.

SureSTART GOLD-GRADE SureSTOP vials SCREW

Sample Handling SureSTART Polypropylene Vials

Oligonucleotides tend to form adducts with residual metal from the glass causing loss of recovery and robustness over time. To ensure the robustness of your method, Thermo Scientific™SureSTART™ Polypropylene Vials should be used for oligonucleotide analysis.
For non-oligo biotherapeutics products, it's important to not only achieve the best sensitivity, but also assure robustness for small injection volumes. Thermo Scientific™ SureSTART™ High RecoveryGlass Snap Top Microvials maximize injection volumes whenanalyzing <2 mL samples.

SureSTARTTM 2 mL Polypropylene Screw Top Microvials SureSTARTTM High Recovery Glass Snap Top Microvials for <2 mL Samples

Sample Prep: mapping and quantitation

SMART Digest™ RNase Kits achieve a controlled mRNA digestion with high reproducibility, and high levels of data quality in an ideal length for sequencing by HRAM-MS.
After SMART Digest™ pepsin kits provide rapid and efficient protein digestion, significantly reducing processing times compared to traditional in-solution methods for peptide mapping and quantitation.

SMART Digest™ RNase Kits SMART Digest™ Pepsin Kits

Oligonucleotide HPLC Columns

Exceptionally high resolution of +1 and -1 nucleic acids, including isomers

These columns are used in HPLC and UHPLC experiments. What sets Thermo Scientific™ DNAPac™ columns apart from the rest is their ability to provide exceptionally high resolution separations of nucleic acids (+1 and -1), including isomers, which is not possible with reversed phase columns. The larger i.d. columns are popularly used for the small scale purification of nucleic acids.

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DNAPac Family of Columns

Superior Oligonucleotide Analysis.

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Protein therapeutics, a class of biotherapeutics, offer advantages over small-molecule drugs by targeting specific functions with reduced adverse effects and immune responses. Analytical approaches and workflows are essential for comprehensive characterization, ensuring product safety and efficacy throughout development and manufacturing.

Catalog Number 15156844

Thermo Scientific™ MAbPac™ Protein A Antibody Analysis and Purification HPLC Column, 12 μm, 4 mm x 35 mm

Catalog Number 10146081

Thermo Scientific™ Hypersil GOLD™ Peptide HPLC Column

Catalog Number 6183639

Thermo Scientific™ MAbPac™ HIC-10 HPLC Column

Catalog Number 7229290

Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC Columns

Catalog Number 6017000

Catalog Number 7237230

Catalog Number 7237229

Catalog Number 6366080

HPLC Columns for Intact Mass & Subunit Analysis

Ideal for intact protein and subunit analysis
Reversed phase (RP) chromatography

MAbPac RP

Affinity Chromotography HPLC Columns

Purify mAbs
Affinity chromatography

MAbPac Protein A

HPLC & UHPLC Columns for Peptide Mapping & MAM

Perform peptide mapping and Multi-Attribute Methods (MAM)
Reversed phase (RP) chromatography

Hypersil GOLD peptide

HIC HPLC columns for the orthogonal analysis of mAbs and related protein therapeutics

Three different chemistries to separate intact mAbs, fragments, aggregates, oxidized mAbs as well as ADC's and bispecifics.
Hydrophobic interaction chromatography (HIC)

MAbPac HIC-10 MAbPac HIC-20 MAbPac HIC-Butyl

Charge Variant Analysis HPLC columns

Detect therapeutic protein modifications
Charge variant analysis by ion-exchange chromatography (IEC)

ProPac 3R SCX ProPac 3R SAX ProPac Elite WCX CX-1 pH Buffers

HILIC HPLC Columns for Glycan Analysis

Perform glycan analysis of neutral monoclonal antibodies and proteins
Hydrophilic interaction liquid chromatography (HILIC)

Accucore 150 Amide HILIC

Sample Handling SureSTART GOLD-grade

Hydrophobic proteins and peptides tend to create salt adduct swith glass vials over time, reducing the recovery of certain proteins or peptides.
Thermo Scientific SureSTART GOLD-grade vials have an ultra-low adsorption glass surface that enables trace-level analysis or strongly adsorbing analytes like proteins.

SureSTART GOLD-GRADE SureSTOP vials SCREW

Sample Handling SureSTART Micovial

For biotherapeutics products, it’s important to not only achieve the best sensitivity, but also assure robustness for small injection volumes.
Thermo Scientific SureSTART high recovery glass snaptop microvials maximize injection volumes when analyzing <2 mL samples, and with their inner V-shaped bottom, enable a residual volume of 4 μL.

SureSTART High Recovery Glass Snap Top Microvials for <2 mL Samples SureSTART 1.7 mL High Recovery Glass Screw Top Microvials for <2 mL Samples

Sample Prep: Peptide Mapping

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

SMART Digest Trypsin Kits

Monoclonal Antibody HPLC Columns

Characterization of mAbs and their aggregates, fragments, and titers

Extensive development of protein and monoclonal antibody (mAb) therapeutics currently underway requires innovative and efficient tools that characterize and quantify structural variants and modifications, monitor product stability and demonstrate safety and efficacy. These specifically designed Thermo Scientific™ MAbPac™ columns provide a full range of high resolution, high efficiency mAb analyses by LC and LC-MS.

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BioLC Columns

Innovative solutions for mAb analysis and characterization.

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MAbPac RP Ordering Guide

Right-sized for highest sensitivity.

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MAbPac RP Columns

Increased sensitivity and throughput with easy-to-connect capillary MAbPac RP columns.

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Protein HPLC Columns

Exceptionally high resolution, high efficiency protein characterization

High resolution and high efficiency set these columns apart. For excellent analytical characterization of proteins, we offer a full range of Thermo Scientific™ affinity, ion exchange, reversed phase, hydrophobic interaction, and size exclusion columns for your protein sample.

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Glycan HPLC Columns

Exceptional characterization, purification and isolation

Mixed-mode Thermo Scientific™ GlycanPac™ columns provide uniquely exceptional high resolution separation of neutral and charged glycans. In addition, we offer traditional amide-HILIC columns for the separation of neutral glycans, and ligand exchange columns for the purification and isolation of carbohydrates using an affinity support.

See Your Biotherapeutics in High Resolution

High Resolution DNA/RNA Separation

AAV- Gene Therapies

Exceptionally high resolution of +1 and -1 nucleic acids, including isomers

HPLC Columns for Intact Mass & Subunit Analysis

Affinity Chromotography HPLC Columns

HPLC & UHPLC Columns for Peptide Mapping & MAM

HIC HPLC columns for the orthogonal analysis of mAbs and related protein therapeutics

Charge Variant Analysis HPLC columns

HILIC HPLC Columns for Glycan Analysis

Sample Handling SureSTART GOLD-grade

Sample Handling SureSTART Micovial

Sample Prep: Peptide Mapping

Characterization of mAbs and their aggregates, fragments, and titers

Exceptionally high resolution, high efficiency protein characterization

Exceptional characterization, purification and isolation

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