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    1. Home
    2. Cell Culture

    Cell Culture

    Cell culture plays an important role in biology and pharmacological research and manufacturing. Each cell culture workflow step, including seeding, cell expansion, harvesting, detection, analysis, and storage, requires a wide range of products that help complete delicate processes and enable strong cell growth.

    Overview
    1. Sample Preparation
    2. Cell Expansion
    3. Harvesting
    4. Detection
    5. Analysis
    6. Storage
    Related Resources
    Other Applications

    Cell Culture

    1. Sample Preparation


    Preparing samples for PCR or qPCR typically requires extraction and purification of nuclear material. Depending on the sample source, this may involve mechanical manipulation via homogenization or require the use of resins or beads to help bind the nucleic acids prior to purification. Prepare your samples with the help of DNA and RNA extraction, isolation, and purification kits, plus reagents, buffers, and more.

    Top Categories

    Cell Lines

    Cell Lines

    Serological Pipets

    Serological Pipets

    Dissociation Reagents

    Dissociation Reagents

    Featured Products

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    Catalog Number 11523127

    Gibco™ StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool
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    Catalog Number 4525485

    Thermo Scientific™ Nunc™ Serological Pipettes
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    Catalog Number 161515

    Fisherbrand™ SureOne™ Aerosol Barrier Pipette Tips
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    Resources

    AcroPrep Filter Plates

    Filename
    25524_AcroPrep_Filter_Plates.pdf
    Size
    13 MB
    Format
    application/pdf

    Benchtop Instrument Portfolio

    Filename
    25524_Benchtop_Instrument_Portfolio.pdf
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    2 MB
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    application/pdf

    Cell Culture Workflow

    Filename
    25524_Cell_Culture_Workflow.pdf
    Size
    1 MB
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    application/pdf

    Essentials for Life Science

    Filename
    25524_Essentials_for_Life_Science.pdf
    Size
    1 MB
    Format
    application/pdf

    Pall AcroPrep Cell Clarification and Sterile Filter Plates

    Filename
    25524_Pall_AcroPrep_Cell_Clarification_and_Sterile_Filter_Plate.pdf
    Size
    5 MB
    Format
    application/pdf

    Thermo Scientific Sorvall BIOS Centrifuge Series

    Filename
    25524_Thermo_Scientific_Sorvall_BIOS_Centrifuge_Series.pdf
    Size
    1 MB
    Format
    application/pdf

    Gibco Cell Culture Basics Handbook

    Filename
    25524_gibco-cell-culture-basics-handbook.pdf
    Size
    8 MB
    Format
    application/pdf

    2. Cell Expansion


    Your next step, cell expansion, is necessary for cell growth and will take place in a vessel containing a culture medium. There are multiple options available based on the cell line being studied. To prepare, shop products for cell expansion including media, sera, and flasks for your research. For more information on these items, download the related resources.

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    Cell Culture Media

    Cell Culture Media

    Sera

    Sera

    Cell Culture Flasks

    Cell Culture Flasks

    Featured Products

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    Catalog Number 7214997

    Corning™ CellBIND™ HYPERStack™ Cell Culture Vessels - New Design
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    Catalog Number 7058048

    Cytiva HyClone™ FetalClone™ I Serum (U.S.)
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    Catalog Number 12359802

    Cytiva Fetal Bovine Serum, US Characterized
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    Resources

    Cleaning and Disinfection in GMP a Environment

    Filename
    Cleaning_and_Disinfection_in_GMP_Environment.pdf
    Size
    526 KB
    Format
    application/pdf

    Introducing the new, aliquot-free Gibco™ One Shot™ FBS 50mL bottle

    Filename
    Gibco-fbs-one-shot-brochure.pdf
    Size
    9 MB
    Format
    application/pdf

    Thermo Scientific Aqua-Tec Water Preservation Cell

    Filename
    Thermo_Scientific_Aqua-Tec_Water_Preservation_Cell.pdf
    Size
    216 KB
    Format
    application/pdf

    Thermo Scientific Forma Steri-Cycle Cleanroom Incubators

    Filename
    Thermo_Scientific_Forma_Steri-Cycle_Cleanroom_Incubators.pdf
    Size
    3 MB
    Format
    application/pdf

    Thermo Scientific Heracell Cleanroom Incubators

    Filename
    Thermo_Scientific_Heracell_Cleanroom_Incubators.pdf
    Size
    3 MB
    Format
    application/pdf

    Smart Note: What is a Certified Cleanroom Compatible Incubator

    Filename
    What_is_Certified_Cleanroom_Compatible_Incubators.pdf
    Size
    759 KB
    Format
    application/pdf

    3. Harvesting


    Collecting and isolating cells may involve centrifugation, depth filtration, and cell detachment. Before proceeding, adherent cells should be detached from the surface of the cell culture flask or dish. Choose to harvest your cells with products from our portfolio. Find cell scrapers, lifters, and reagents, as well as resources to support your workflow.

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    Cell Scrapers

    Cell Scrapers

    Cell Line Lysates

    Cell Line Lysates

    Dissociation Reagents

    Dissociation Reagents

    Featured Products

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    Catalog Number 3821025

    Fisherbrand™ Cell Scrapers
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    Catalog Number 4919324

    Gibco™ TrypLE™ Express Enzyme (1X), no phenol red
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    Catalog Number 11577692

    Fisherbrand™ Cell Lifters
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    Resources

    Thermo Scientific Herasafe 2025 Biological Safety Cabinet

    Filename
    Thermo_Scientific_Herasafe_2025_Biological_Cabinet.pdf
    Size
    2 MB
    Format
    application/pdf

    Thermo Scientific Medifuge Centrifuge

    Filename
    Thermo_Scientific_Medifuge_Centrifuge.pdf
    Size
    947 KB
    Format
    application/pdf

    4. Detection


    Now that your cells are harvested, determine how you’ll read your results. There are many equipment options available to make this process more accurate and efficient. Buy counters, readers, and more to detect cells and cell colonies. Learn about microplate selection and imaging with our additional resources.

    Top Categories

    Colony Counters

    Colony Counters

    Microplate Readers and Accessories

    Microplate Readers and Accessories

    Cell Culture Microplates

    Cell Culture Microplates

    Featured Products

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    Catalog Number 16832556

    Invitrogen™ Countess™ 3 FL Automated Cell Counter
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    Catalog Number 4672156

    Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader
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    Catalog Number 15813915

    Corning™ Cell Counter
    Viewing 1-3 of 3

    Resources

    AcroPrep Filter Plates

    Filename
    AcroPrep_Filter_Plates.pdf
    Size
    13 MB
    Format
    application/pdf

    Agilent BioTek Multimode Microplate Readers

    Filename
    Agilent_BioTek_Multimode_Microplate_Readers.pdf
    Size
    2 MB
    Format
    application/pdf

    Agilent BioTek Selection Guide

    Filename
    Agilent_BioTek_Selection_Guide.pdf
    Size
    2 MB
    Format
    application/pdf

    5. Analysis


    Begin to study your cells through observation and analysis. With the right equipment, you can make this step of your workflow more effective. Choose from a variety of microscopes and microplate washers and readers for your cell culture analysis. For more information, browse our brochures and buying guides or visit our cell analysis page.

    Top Categories

    Microscopes

    Microscopes

    Cell Based Assays

    Cell Based Assays

    Cellular Imaging

    Cellular Imaging

    Featured Products

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    Catalog Number 15918206

    Invitrogen™ EVOS™ M7000 Imaging System
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    Catalog Number 7163847

    Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader
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    Catalog Number 6070001

    Thermo Scientific™ Wellwash™ Versa Microplate Washer
    Viewing 1-3 of 3

    Resources

    Agilent BioTek Multimode Microplate Readers

    Filename
    Agilent_BioTek_Multimode_Microplate_Readers.pdf
    Size
    2 MB
    Format
    application/pdf

    6. Storage


    There are many product options and techniques to choose from when storing your cells. Cryopreservation, for example, helps you keep cells safe long term for future research projects. Find vials, microplates, and media for cell storage. Make these products part of your workflow by learning more and placing an order.

    Top Categories

    Cryogenic Storage Vials

    Cryogenic Storage Vials

    Cryogenic Equipment

    Cryogenic Equipment

    Transport and Preservation Media

    Transport and Preservation Media

    Featured Products

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    Catalog Number 4526104

    Thermo Scientific™ Nunc™ Biobanking and Cell Culture Cryogenic Tubes
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    Catalog Number 4530722

    Thermo Scientific™ Locator™ Plus Rack and Box Systems
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    Catalog Number 13493809

    Cytiva HyClone™ Cryopreservation Media
    Viewing 1-3 of 3

    Resources

    Thermo Scientific Sample Storage Solutions

    Filename
    Thermo_Scientific_Dample_Storage_Solutions.pdf
    Size
    5 MB
    Format
    application/pdf

    Related Resources


    How to Reduce Hidden Costs in the Cell Culture Lab

    Cell Culture Workflow, Growth, Analysis, Processing

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    Cell Culture: BioPharma Application

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    Other Applications


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