Invitrogen™ O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (RL2)

Mouse Monoclonal Antibody

MA1-072 detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, virus and rat tissues. MA1-072 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with MA1-072 primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus. The MA1-072 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.