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Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli Cells, 96-well plate

Código de producto. 31781242
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Cantidad:
1 x 96 Well Plate (20 μL/Well)
Tamaño de la unidad:
cada uno
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31781242 1 x 96 Well Plate (20 μL/Well) cada uno
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Código de producto. 31781242 Proveedor Invitrogen™ N.º de proveedor A40007058

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Descripción

MultiShot TOP10 Chemically Competent E. coli Cells provide high-efficiency transformation for routine cloning in an automation-ready 96-well format. Ideal for stable propagation of high-copy plasmids and blue/white screening in high-throughput workflows.

Invitrogen MultiShot TOP10 Chemically Competent E. coli Cells are derived from DH10B cells and are one of the most popular laboratory strains. Provided in a clear skirted 96-well microtiter plate to simplify high-throughput workflows and provide transformation efficiencies of >1 x 108 cfu/μg control plasmid DNA, Multishot TOP10 cells are a versatile strain that can be used in multiple routine cloning applications.

Features of MultiShot TOP10 competent cells include:

  • TOP10 strain ideal for high-efficiency cloning and plasmid propagation
  • Microtiter plate format design convenient for automated high-throughput cloning
  • mcrA mutation that supports efficient transfection of methylated DNA
  • lacZΔM15 allele that permits rapid blue/white screening of transfectants
  • endA1 mutation that helps reduce endonuclease activity and improve plasmid DNA yields
  • recA1 mutation helps reduce unwanted recombination

MultiShot TOP10 competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.

Genotype

F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

TRUSTED_SUSTAINABILITY

Especificaciones

Tipo de producto Chemically Competent Cells
Incluye pUC19 DNA, S.O.C media
Nivel de eficiencia de transformación ≥1.0 x 108
Condiciones de envío Dry Ice
Especie E. coli
Formato 96-well Plate
Línea de productos MultiShot
Para utilizar con (aplicación) Cloning
Cantidad 1 x 96 Well Plate (20 μL/Well)

For Research Use Only.

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