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  7. 10217112

Invitrogen™ Rhod-2, AM, cell permeant

Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells

Brand:  Invitrogen™ R1244

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Product Code. 10217112

  • 830.00€ / 1 miligramo

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Description

Description

Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.

  • Fluorescence signal from these cells is generally measured using fluorescence microscopy
  • Label (Ex/Em of Ca2+-bound form): Rhod-2 (552/581nm)
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in the absence of Mg2+, in buffer: approximately 570nM
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
  • Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store in freezer -5°C to -30°C and protect from light.
Cell Viability and Proliferation
Stain
1mg
Fluorescence
Fluorescence Microscope
Fluorescent Dye-Based
Room Temperature
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Manuals

Preparation of Dihydrorhod-2 AM Version: 01-31-2001 File size: 160.6 kB
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2876757 Certificate TypeCertificate of Analysis Date26/03/2024 Catalog NumberR1244
Lot Number2790053 Certificate TypeCertificate of Analysis Date18/10/2023 Catalog NumberR1244
Lot Number2712239 Certificate TypeCertificate of Analysis Date21/06/2023 Catalog NumberR1244
Lot Number2495444 Certificate TypeCertificate of Analysis Date06/10/2022 Catalog NumberR1244
Lot Number2509819 Certificate TypeCertificate of Analysis Date19/05/2022 Catalog NumberR1244
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Invitrogen™ Rhod-2, AM, cell permeant > 1mg

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For Research Use Only. Not for use in diagnostic procedures.