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Description
Consiga una separación de los oligonucleótidos por LC y LC-MS de alta resolución, incluidos los fragmentos de ADN/ARN bicatenarios (ds), utilizando estas columnas de fase reversa DNAPac.
Consiga separaciones de oligonucleótidos de fase reversa superiores con las columnas para HPLC Thermo Scientific™ DNAPac™ RP. La química exclusiva está diseñada para el análisis de oligonucleótidos y fragmentos de ADN/ARN de doble cadena (ds) mediante LC-UV o LC-MS. La química de las columnas proporciona un rendimiento excelente bajo una amplia gama de composiciones de pH, temperatura y fase móvil. Además, el ancho tamaño de poro de la resina proporciona una excelente separación de ácidos nucleicos de doble cadena grandes de hasta 10 k de pares de bases.
DNAPac RP reversed phase (RP) column is based on spherical wide-pore 4 μm polymer resin specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is highly stable to extreme pH (0-14), and temperature (5-110°C) and fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of small to large nucleic acids with low carry-over. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments:
- Designed for ion-pair reversed-phase (IP-RP) LC and LC/MS separations of both oligonucleotides and double-stranded nucleic acids
- High resolution and high throughput
- Excellent MS compatibility
- Wide operating pH range (0-14) and high temperature stability (up to 110°C)
- Biocompatible hardware to eliminate analyte absorption
Specifications
Specifications
| Formato de columna | Columna preparativa, estándar |
| Diámetro (métrico) | 21,2 mm |
| Caudal | De 4 a 8 ml/min |
| Longitud (métrico) | 150 mm |
| Presión máxima | 2900 psi (200 bar) |
| Tamaño de partículas | 4 μm |
| pH | De 0 a 14 |
| Cantidad | Unitary |
| Temperatura | up to 110°C |
| Fase estacionaria | Patentado |
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