Enzimas e inhibidores

Invitrogen™ TURBO DNA-free Kit

Ideal tool for clearing residual DNA TURBO DNAFREE 50 RXNS

Invitrogen™ Clonase™ Gateway™ LR Clonase II Enzyme Mix

Enzymes and buffer in single mix LR CLONASE II ENZYME MIX

Thermo Scientific™ ADN ligasa T4

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. T4 DNA LIGASE 5U/UL 5X1000U

Invitrogen™ Clonase™ Gateway™ BP Clonase II Enzyme Mix

Catalyzes in vitro recombination of PCR products BP CLONASE II ENZYME MIX

Thermo Scientific™ Seroalbúmina bovina (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. BSA 20MG/ML 5MG

Thermo Scientific™ Tampón TBE 10X (Tris-borato-EDTA)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA). TBE BUFFER 1L 10X

Thermo Scientific™ Polinucleótido quinasa T4 (PNK T4)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA. T4 PNK 2500U 10U/UL

Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST ECORI 500UL

Invitrogen™ T4 DNA Ligase (1U/μL)

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA T4 DNA LIGASE

Thermo Scientific™ RNase A, DNase and protease-free

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A. RNASE A DNASE FRE 10MG/ML 10MG10MG

Thermo Scientific™ Tampón TAE 50X (Tris-acetato-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ XhoI

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI). XHOI 10U/UL 5000U

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 150RXN Thermo Scientific FastDigest NotI restriction enzyme recognizes GC¬GGCCGC site

Thermo Scientific™ ADNasa I, sin ARNasa

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups. DNASE I RNASE-FRE 50U/UL 1000U000U

Thermo Scientific™ NcoI

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I). NCOI 10U/UL 2500U

Thermo Scientific™ Inhibidor de ARNasa RiboLock

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions. RIBOLOCK RI 40U/UL 24X2500U

Thermo Scientific™ DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI). DPNI 10U/UL 1000U

Invitrogen™ DNase I, Amplification Grade

Purified and tested for nondetectable levels of RNase contamination DNASE I AMP GRADE

Thermo Scientific™ NotI

The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). NOTI 50U/UL 1500U

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

50MG Proteinase K, from Tritirachium album, DNaseand RNase free, for Molecular Biology

Thermo Scientific™ PstI

The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). PSTI 10U/UL 5X3000U

Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 10U/UL 10000U

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

Invitrogen™ TURBO™ DNase (2U/μL)

Efficiently degrades and digests DNA TURBO DNASE 5 X 1000 U

Invitrogen™ DNA-free™ DNA Removal Kit

Safely eliminate DNA contamination from RNA samples DNA-free

Thermo Scientific™ SalI

The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer. SALI 10U/UL 5X1500U

Thermo Scientific™ BpiI (BbsI)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I). BPII (BBSI) 10U/UL 1000U

Invitrogen™ T4 DNA Ligase (5U/uL)

Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini T4 DNA LIGASE HI CONC.

Thermo Scientific™ FastDigest™ BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST BAMHI 500UL

Thermo Scientific™ FastDigest™ SpeI (BcuI)

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI). FASTDIGEST SPEI (BCUI) 50UL

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