Learn More
Descripción
Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.
Features of TaqMan Gene Expression assays include:
- Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
- Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
- Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
- Accurate—identify small fold-changes with high accuracy of quantitation
- Extensive content—Over 1.8 million predesigned assays available for over 25 different species
- Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
- Fast delivery—your assays shipped in as little as 1–2 days (see below)
Approximate ship time
- Inventoried: 1–2 days in North America, 3–5 days in Europe*
- Made to order: 4–6 days in North America, 6–10 days in Europe
TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.
Pre-formulated assay
- 2 unlabeled PCR primers (900 nM each final 1X concentration)
- 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)
All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.**
Recommended master mix: TaqMan Fast Advanced Master Mix (Cat. No. 4444556)
*Our most popular assays are stocked in North America and Europe for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.
**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.
Especificaciones
Especificaciones
| Concentración | 60X |
| Contenido y almacenamiento | 1 tubo que contiene un mezcla de ensayo preformulado 20X (tamaños XS, S y M) o 60X (tamaño L) (1 sonda y 2 primers). Almacenar entre -15 °C y -25 °C. |
| Método de detección | Sonda de cebado |
| Formato | Tubo |
| GC-Rich PCR Performance | Alto |
| Método de PCR | qPCR |
| Velocidad de reacción | Fast |
| Para utilizar con (equipo) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Línea de productos | TaqMan |
| Tipo de producto | Ensayo de expresión génica |
| Show More |
Safety and Handling
- TAQMAN GENE EX ASSAYS INV, XS
- Peligro
- Carcinogenicidad Categoría 2
- Toxicidad para la reproducción Categoría 1B
- Toxicidad específica para determinados órganos Categoría 2
- H351-Se sospecha que provoca cáncer.
- H360-Puede perjudicar la fertilidad o dañar al feto.
- H373-Puede provocar daños en los órganos.
- P201-Pedir instrucciones especiales antes del uso.
- P202-No manipular la sustancia antes de haber leído y comprendido todas las instrucciones de seguridad.
- P260-No respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.
- P280-Llevar guantes/prendas/gafas/máscara de protección.
- P308+P313-EN CASO DE exposición manifiesta o presunta: Consultar a un médico.
- P314-Consultar a un médico en caso de malestar.
- P501b-Eliminar el contenido/el recipiente en conforme a la reglamentación local/regional/nacional/internacional.
- MIXTURE LIST-Contain: formamide
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.