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Descripción
- Gran selección de ensayos de polimorfismos de un solo nucleótido (SNP) avanza los ensayos de validación y cribaje para aplicaciones de asociación de enfermedades y estratificación de poblaciones
- Fácil de usar, formato de un solo tubo
- El diseño robusto del ensayo proporciona resultados muy precisos, reproducibles y fiables
- Un flujo de trabajo sencillo permite obtener resultados rápidos
Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.
Benefits:
- Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
- Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
- Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
- Tested—all human SNP genotyping assays ordered with VIC/FAM dye labels are functionally tested to ensure allelic discrimination. Assays ordered with Cyanine 5/ABY dyes are not functionally tested.
- Multiplexing ready—TaqMan assays are now available with with VIC/FAM or Cyanine 5/ABY formats to support simultaneous detection of two sets of SNPs (two pairs of alleles).
Approximate ship time
4–6 days in North America and 6–10 days in Europe (VIC/FAM); 4-10 days in North America, 6-14 days in Europe (Cyanine 5/ABY)
TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (singleplex) or TaqPath ProAmp Master Mix (multiplex) (sold separately).
All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.
All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*
Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355) or TaqPath ProAmp Master Mix (Cat. No. A30865)
*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.
Especificaciones
Especificaciones
| Concentración | 80X |
| Contenido y almacenamiento | •1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers). •Store at -15°C to -25°C. |
| Método de detección | qPCR |
| Formulario | Liquid |
| Formato | Tube |
| Método de PCR | qPCR |
| Velocidad de reacción | Fast |
| Para utilizar con (equipo) | 7500 System, 7500 Fast System, 7900HT System, StepOne, StepOnePlus, ViiA 7 System, QuantStudio Absolute Q Digital PCR System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals. |
| Modificación de sonda interna | Cyanine 5 - MGB probe detects Allele 1, ABY - MGB probe detects Allele 2 |
| Etiqueta o tinte | ABY, Cy5 |
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
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