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Gibco™ RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Marca: Gibco™ 42402016
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Descripción
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
- With: Phenol Red, HEPES
- Without: L-glutamine
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Use
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Especificaciones
| Normas ISO 13485, FDA | |
| CO2 | |
| Libre de material de origen animal | |
| Cultivo de células de mamíferos | |
| RPMI 1640 | |
| Estéril con filtro | |
| Sin glutamina, Sin piruvato sódico | |
| Embalaje sostenible | |
| 270 - 300 mOsm/kg | |
| Gibco™ | |
| Temperatura ambiente |
| Condiciones de almacenamiento: De 2 a 8° C. Proteger de la luz. Condiciones de envío: Ambiente. Vida útil: 12 meses a partir de la fecha de fabricación. |
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| Medio Roswell Park Memorial Institute (RPMI) 1640, 500 ml, líquido, concentración 1X, pH de 7,0 a 7,4, con rojo de fenol, HEPES, 12 meses de vida útil, osmolalidad de 270 a 300 mOsm/kg, para cultivo de células de mamíferos | |
| 1 X | |
| Líquido | |
| Suplementos de suero estándar | |
| Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES), Rojo de fenol, Bicarbonato sódico | |
| Cultivo de células de mamíferos | |
| cGMP-compliant under the ISO 13485 standard | |
| entre 7 y 7,4 | |
| 500 mL |
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.