Thermo Fisher Scientific™ ReadyProbes™ Hydrophobic Barrier Pap Pen

• The ReadyProbes Hydrophobic Barrier Pap Pen creates a water-repellent barrier when used on a microscope slide or coverslip
• By lightly drawing a rectangle or circle around the tissue section or cells on the microscope slide, aqueous incubation reagents can be kept inside the circle or rectangle
• Save precious reagents by incubating with less volume
• The pen can also be used to keep reagents separate when more than one tissue section is mounted on a microscope slide
• The light blue barrier line is heat resistant and compatible with most immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) reagents, including detergent-containing reagents
• It is insoluble in alcohol and acetone, but can be removed with clearing agent (e.g., xylene)
• When applied as directed, the nib provided with each pen will create a 3 mm wide line
• The pen design ensures the nib can move and that the nib is not adhered to the pen itself
• In some instances, during shipping, the nib may become dislodged and fall out. Simply put the nib back into the neck of the pen and use as per the supplied instructions
• The pen residue does fluoresce – apply the Hydrophobic Barrier a few mm away from tissue section or cells, so it does not interfere with imaging

How to Use

• Dab the nib of the pen on a paper towel, to make sure the flow of the hydrophobic fluid is uninterrupted
• Lightly trace a circle or rectangle around the tissue/cells 2-4 times, with a thick line, while keeping a few millimeters distance from the edge of the tissue/cells
• Let the line dry for 20-30 minutes at room temperature
• Repeat the process, if needed