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Rat brain tissue lysate, Non-denatured; Abnova

Product Code. 16039605
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Cantidad:
200 μg
Unit Size:
200 microgramos
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Product Code. Cantidad Unit Size
16039605 200 μg 200 microgramos
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Product Code. 16039605 Supplier Abnova Supplier No. L041W2.200ug
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Fecha estimada de envoi: 19-10-2026
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Description

  • Tissue: Brain
  • Host: Rat
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

Para utilizar con (aplicación) Immunoprecipitation, Western Blot
Tissue Brain
Descripción Whole tissue lysate (non-denatured)
Tampón de lisis Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Método de preparación Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Pruebas de control de calidad 12.5% SDS-PAGE Stained with Coomassie Blue.
Cantidad 200 μg
Tampón de almacenamiento In modified RIPA Lysis Buffer.
Especie del huésped Rat
Concentración 5 mg/mL
Contenido y almacenamiento Almacenar a -80°C. Dividir para evitar repetir la congelación y descongelación.
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