Invitrogen™ ProcartaPlex™ Human Triiodothyronine (T3) Competitive Simplex Kit

A ProcartaPlex Basic Kit (sold separately) is required to run the assay, as Simplex kits include only assay-specific components and do not contain any buffer or plate.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex assays are bead-based immunoassays designed for multiplex protein analysis on Luminex™ xMAP instruments. They enable the simultaneous detection and quantification of multiple protein targets from a single, small-volume sample, helping researchers save time, sample, and cost compared to traditional single-analyte ELISAs.

Depending on the analyte, ProcartaPlex assays are based on either sandwich ELISA or competitive immunoassay principles.

• Sandwich assays use two highly specific antibodies binding to different epitopes of the target protein.
• Competitive assays measure small molecules or analytes with limited epitopes, where the sample analyte competes with a labeled tracer for antibody binding, resulting in an inverse signal.

How the technology works

ProcartaPlex assays use Luminex xMAP technology, in which microscopic magnetic beads are internally dyed with precise ratios of red and infrared fluorophores. Each bead population has a unique spectral signature and is coated with antibodies specific to a single target. This allows different analytes to be measured in parallel within the same well.

Unlike traditional ELISA, where antibodies are immobilized on a plate, ProcartaPlex reagents are free-floating in solution, improving reaction kinetics and enabling efficient multiplexing with the following benefits:

• Multiplexing efficiency—measure up to 80 protein targets simultaneously
• Low sample volume—as little as 25 μL for serum or plasma, or 50 μL for cell culture supernatant
• Time-saving workflow—results in approximately four hours
• High Throughput - 96-well and even 384-well assay formulations are available helping conserve valuable samples while increasing assay efficiency.

ProcartaPlex multiplex panels and simplex assays are available for six (species: human, mouse, rat, nonhuman primate, porcine, and canine).

For more information and a full list of available targets, visit thermofisher.com/procartaplex.