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PowerUp™, SYBR™ PowerUp™ SYBR™ Green Master Mix

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

131.00€ - 2490.00€

Especificación

Método de detección Detection Method: SYBR; PCR Method: qPCR
Velocidad Fast or Standard
Tipo de muestra DNA
Para utilizar con (equipo) 7300 System, 7500 Fast Dx System, 7500 Fast System, 7700 System, 7900HT Fast System, Agilent Mx3005P system, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, Roche LightCycler 480, Roche LightCycler 96, StepOne™ System, StepOnePlus™ System, ViiA™ 7 System
Para utilizar con (aplicación) Real time PCR (qPCR)
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Productos 8
Código de producto Marca N.° de reacciones Precio Cantidad y disponibilidad  
Código de producto Marca N.° de reacciones Precio Cantidad y disponibilidad  
15350929 Applied Biosystems™
A25742
500 Reactions 312.00€
5 ml Ver productos alternativos
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Fecha de envío estimada
del proveedor 01-04-2019
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15360929 Applied Biosystems™
A25743
5000 Reactions 2470.00€
50 ml Ver productos alternativos
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En stock 12
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15310939 Applied Biosystems™
A25778
5000 rxn(s) 2490.00€
paquete de 10 Ver productos alternativos
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Fecha de envío estimada
del proveedor 11-04-2019
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15330939 Applied Biosystems™
A25780
500 rxn(s) 317.00€
paquete de 5 Ver productos alternativos
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En stock 137
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15340939 Applied Biosystems™
A25918
1000 rxn(s) 586.00€
paquete de 10 Ver productos alternativos
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En stock 68
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15380929 Applied Biosystems™
A25776
1000 rxn(s) 574.00€
paquete de 2 Ver productos alternativos
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Fecha de envío estimada
del proveedor 02-04-2019
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15390929 Applied Biosystems™
A25777
2500 rxn(s) 1318.00€
paquete de 5 Ver productos alternativos
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Fecha de envío estimada
del proveedor 29-03-2019
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15366158 Applied Biosystems™
A25779
200 Reactions 131.00€
paquete de 2 Ver productos alternativos
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En stock 344
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Descripción

Descripción

  • A dual hot-start mechanism for excellent specificity
  • Highly reproducible CTs over a broad dynamic range
  • Inclusion of UDG to help prevent carryover contamination
  • Stability of pre-assembled reactions for up to 72 hours
  • Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:
  • Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
  • PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
  • In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:
  • PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
  • In addition, this master mix provides efficient amplification over 6 logs of sample input
  • Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:
  • The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:
  • The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
  • This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:
  • PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
  • It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
  • For optimal results, recommended primer concentrations are 300 to 800nM

Order Info

Shipping Conditions: Wet Ice

Especificación

Especificación

Detection Method: SYBR; PCR Method: qPCR
DNA
Real time PCR (qPCR)
Fast or Standard
7300 System, 7500 Fast Dx System, 7500 Fast System, 7700 System, 7900HT Fast System, Agilent Mx3005P system, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, Roche LightCycler 480, Roche LightCycler 96, StepOne™ System, StepOnePlus™ System, ViiA™ 7 System
Store at 2° to 8°C.

For Research Use Only. Not for use in diagnostic procedures.