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  6. Thermo Scientific™ POROS™ CaptureSelect™ LC Lambda Affinty HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ LC Lambda Affinty HPLC Columns

Código de producto. 18436818 Tienda Thermo Scientific Productos
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4.6 x 100 mm
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Código de producto. 18436818 Proveedor Thermo Scientific™ N.º de proveedor 4469150

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Descripción

Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

POROS CaptureSelect LC Lambda HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands. The combination allows three minute quantitation of Fabs, immunoglobulins, and bispecific antibodies having a Lambda light chain.

Features:

  • Broad utility—POROS A, G and CaptureSelectaffinity ligands address most biological drug classes
  • Rapid—POROS resins offer fast quantitation or purification of immunoglobulins and fusion proteins
  • Hands-off operation—HPLC method can be automated for streamlined operation
  • Robust—deliver high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity

The combination of CaptureSelect LC Lambda affinity ligands when combined with POROS 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of all immunoglobulins and Fabs having a Lambda light chain (all subclasses).

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.

Automation

POROS affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

LC Lambda Column Specifications:

  • Protein Binding Capacity: 10 mg/mL
  • Species Specificity: Human
  • Column Material: PEEK™ polymer or stainless steel
  • Frit: 2 μm, titanium
  • POROS Resin: Cross-linked poly(styrenedivinylbenzene)
  • CaptureSelect Ligands: Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae
TRUSTED_SUSTAINABILITY

Especificaciones

Formato de columna Analytical Column, Biocompatible PEEK
Diámetro (métrico) 2.1 mm
Caudal Hasta 5000 cm/h
Para utilizar con HPLC
Longitud (métrico) 30 mm
Presión máxima 180 bar
Tamaño de partículas 20 μm
pH 1-10
Tamaño de poro 2000 Å
Cantidad 1 columna
Temperatura 2-40°C
Fase estacionaria CaptureSelect™ CL lambda
Contenido y almacenamiento La columna incluye accesorios EZ grip y certificado de prueba de columna

Conservar a 4° C. NO CONGELE ESTOS PRODUCTOS.
Línea de productos POROS
Capacidad de unión 10.0 mg/mL IgG
Volumen de elución máx 200 μl
Volumen de elución mín 100 μl
Reactividad Humano
Compatibilidad de alto rendimiento No compatible con alto rendimiento (manual)
Tipo de muestra Sobrenadantes de cultivo celular
Molécula diana Cadena ligera lambda
Condiciones de envío Hielo húmedo
Matriz Poliestirenodivinilbenceno
Volumen (métrico) 100 μl
Dimensiones 2.1 x 30 mm
Material de embalaje Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
Tipo de columna Affinity, preempaquetada
Material PEEK
Técnica Bio-LC
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For Research Use Only. Not for use in diagnostics procedures.

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