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  6. Thermo Scientific™ POROS™ CaptureSelect™ IgM Affinty HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ IgM Affinty HPLC Columns

Código de producto. 18456818 Tienda Thermo Scientific Productos
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4.6 x 100 mm
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Código de producto. 18456818 Proveedor Thermo Scientific™ N.º de proveedor 4469152

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Descripción

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

POROSCaptureSelect IgM HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands. The combination allows three minute quantitation of IgM antibodies.

Features:

  • Broad utility—POROS A, G and CaptureSelectaffinity ligands address most biological drug classes
  • Rapid—POROS resins offer fast quantitation or purification of immunoglobulins and fusion proteins
  • Hands-off operation—HPLC method can be automated for streamlined operation
  • Robust—deliver high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity

The combination of CaptureSelect IgM affinity ligands when combined with POROS 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of IgM.

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.

Automation

POROS affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

LC Lambda Column Specifications:

  • Protein Binding Capacity: 4 mg/mL
  • Species Specificity: Human, Mouse, Rat
  • Column Material: PEEK™ polymer or stainless steel
  • Frit: 2 μm, titanium
  • POROS Resin: Cross-linked poly(styrenedivinylbenzene)
  • CaptureSelect Ligands™: Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae
TRUSTED_SUSTAINABILITY

Especificaciones

Diámetro (métrico) 2.1 mm
Caudal Up to 5000 cm/hr.
Longitud (métrico) 30 mm
Presión máxima 180 bar
Tamaño de partículas 20 μm
Tamaño de poro 2000 Å
Cantidad 1 columna
Fase estacionaria IgM affinity ligand, human-, mouse-, and rat-specific
Contenido y almacenamiento Column includes EZ grip fittings and column test certificate

Store at 4°C. DO NOT FREEZE.
Línea de productos CaptureSelect, POROS
Capacidad de unión 4 mg/mL
Volumen de elución máx 200 μL
Volumen de elución mín 100 μL
Reactividad Human, Mouse, Rat
Compatibilidad de alto rendimiento Not High-throughput Compatible (Manual)
Tipo de muestra Cell Culture Supernatants
Molécula diana IgM Antibodies
Condiciones de envío Wet Ice
Matriz Polystyrenedivinylbenzene
Volumen (métrico) 0.1 mL
Dimensiones 2.1 x 30 mm
Tipo de columna Affinity
Material PEEK
Tipo Column
Diana de purificación IgM Antibodies
Para utilizar con (aplicación) HPLC
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For Research Use Only. Not for use in diagnostics procedures.

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