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Thermo Scientific™ Pierce™ Agarose ChIP Kit

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Marca:  Thermo Scientific™ 26156

Detalles adicionales : Peso : 0.10000kg Transport : UN number : 1219 Chem class : 3 Pack group : II

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Código de producto. 10220425

  • 588.00€ / cada uno


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Descripción

Descripción

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

  • Simple and streamlined ChIP assay protocol (< 8 hours)
  • Highly efficient isolation and lysis of nuclei
  • Easy and reproducible enzymatic digestion
  • Low-background and high-capacity Protein A/G agarose resin
  • Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
  • Fast and reproducible spin-column format; columns and collection tubes included
  • High-recovery DNA purification
  • Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

Especificaciones

Especificaciones

Pierce Agarose ChIP Kit
DNA Column Wash Solution, 6mL, ChIP Grade Protein A/G Plus Agarose, 0.65mL, Microcentrifuge Tubes (1.5mL), 75 tubes, Pierce Chromatin Prep Module (Part No. 26158), Normal Rabbit IgG (1mg/mL), 10µL, IP Dilution/Wash Buffer (5X), 11mL, DNA Column Elution Solution, 5mL, DNA Column Binding Solution, 30mL, DNA Clean-Up Column, 40 columns, IP Elution Buffer (2X), 4.5mL, Column Accessory Pack, 3 packs, ChIP Positive Control Primers (GAPDH promoter), 100µL, Anti-RNA Polymerase II Antibody, 25µL, IP Wash Buffer 3 (5X), 4.5mL, pH Indicator, 0.8mL
30 complete chromatin IP assay experiments
Multi-component kit
30 rxn kit
Agarose
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