Invitrogen™ pan T cells Monoclonal Antibody (OX-52), FITC

Mouse Monoclonal Antibody

Anti-rat pan T lymphocyte monoclonal antibody immunoprecipitates a two chain structure (95, 120 kDa) largely restricted to T lymphocytes and thymocytes. Applications therefore include the identification of T lymphocyte lineage cells in suspension and tissue. Has previously been used to stain T cell areas of the spleen, lymph nodes and Peyer's patch. In the thymus, labels all thymocytes, however medullary cells are more strongly positive than cortical cells. The antibody does not label such non-lymphoid tissues such as brain, kidney, liver and skin. This antibody stains approximately 1.0 % of bone marrow cells, and 56 % thoracic duct lymphocytes. Weak staining occurs with 50 % dendritic cells from thoracic duct of mesenteric lymphadenectomized rats. The antigen recognized by this antibody is not expressed on granulocytes or macrophages. The function of the antigen recognized by this antibody has not, as yet, been associated with any particular function of T cells. The antibody does not inhibit the allogeneic mixed leukocyte response, nor does it inhibit T cytotoxic effector cell function. This clone works in flow cytometry, Immunoprecipitation and with frozen sections.