missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Reactivos y kits de inmunoensayo
  4. Kits ELISA para investigación en ciencias de la vida
  5. Invitrogen™ Human TNF alpha ELISA Kit 

Invitrogen™ Human TNF alpha ELISA Kit

Código de producto. p-4922264
Cambiar vista
Click to view available options
Cantidad:
2 x 96 pruebas
5 x 96 pruebas
96 pruebas
Tamaño de la unidad:
192 tests
96 tests
paquete de 5
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Código de producto. Cantidad unitSize
10737663 96 pruebas 96 tests
10008932 2 x 96 pruebas 192 tests
10632644 5 x 96 pruebas paquete de 5
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Este artículo no se puede devolver. Vea la política de devoluciones
Código de producto. 10737663 Proveedor Invitrogen™ N.º de proveedor KHC3011
Añadir a la cesta
Edge
Añadir a la cesta
Este artículo no se puede devolver. Vea la política de devoluciones

Descripción

Includes

Human TNF-α Antibody Coated 96-Well Plate
Human TNF-α Standard
Standard Diluent Buffer
Human TNF-α Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
etailed protocol with validation tests

ELISA

The Human Tumor Necrosis Factor alpha (Hu TNFα) ELISA quantitates Hu TNFα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFα. Principle of the method The Human TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Información de órdenes

Condición de envío:Hielo seco

TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso P01375
Intervalo del ensayo 15,6-1000 pg/ml
Sensibilidad del ensayo 1,7 pg
Contenido y almacenamiento Placas prerecubiertas, patrón o calibrador, anticuerpo detector, conjugado de HRP, diluyentes, tampón de lavado, sustratos y solución de parada. Almacene el kit a 2-8 °C. Consulte el manual del producto para conocer el contenido detallado y las condiciones de almacenamiento para obtener la máxima estabilidad.
Conjugado Biotina
Formato del kit de inmunoensayo Sandwich ELISA Kit
Tipo de producto Kit de ELISA
Familia de proteínas Cytokines & Receptors
Tipo de muestra Plasma, Sobrenadante, Suero
Especies diana Humano
Para utilizar con (aplicación) Detección colorimétrica
Para utilizar con (equipo) Lector de microplacas colorimétrico
Alias de gen APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factorAPC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor; DIF-alpha; TNFA; TNFSF2; TNLG1F; TNF
ID de gen (Entrez) 7124
Símbolo de gen DIF-alfa, TNFA, TNFSF2, TNLG1F, TNF
Tiempo de incubación 4 h
CV interensayo 7,5%
CV intraensayo 4,4%
Contenido del kit Placa de 96 pocillos recubiertos previamente, Estándar, Tampón de dilución estándar, Anticuerpo de detección biotinilado, Estreptavidina-HRP, Diluyente HRP, Tampón de lavado, Cromógeno, Solución de parada, Cubiertas adhesivas para placas
Etiqueta o tinte Peroxidasa de rábano picante (HRP)
Gen marcador TNF-α
Cantidad 96 pruebas
Estado normativo RUO
Volumen de muestra Plasma, 100 μl; suero, 100 μl; sobrenadante, 100 μl
Condiciones de envío Wet Ice
Requisitos de almacenamiento De 2 °C a 8 °C
Objetivo Humano
Tiempo de prueba 1 h 20 min
Tiempo total del ensayo 4 h
Mostrar más Mostrar menos

Para uso exclusivo en investigación.No apto para uso en procedimientos diagnósticos.

Título del producto
Seleccione un problema

Al hacer clic en Enviar, acepta que Fisher Scientific se ponga en contacto con usted en relación con los comentarios que ha proporcionado en este formulario. No compartiremos su información para ningún otro fin. Toda la información de contacto proporcionada se mantendrá de acuerdo con nuestra Política de Privacidad. Política de privacidad.