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  5. Invitrogen™ Human MMP2 ELISA Kit 

Invitrogen™ Human MMP2 ELISA Kit

Código de producto. 10328634
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96 pruebas
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Código de producto. 10328634 Proveedor Invitrogen™ N.º de proveedor KHC3081
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Descripción

Includes

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers

ELISA

The Human Matrix MetalloProteinase 2 (Hu MMP2) ELISA quantitates Hu MMP2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP2. Principle of the method The Human MMP2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

Información de órdenes

Condición de envío:Hielo seco

TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso P08253
Intervalo del ensayo 0,78-50 ng/ml
Sensibilidad del ensayo <1 ng/mL
Contenido y almacenamiento Placas prerecubiertas, patrón o calibrador, anticuerpo detector, conjugado de HRP, diluyentes, tampón de lavado, sustratos y solución de parada. Almacene el kit a 2-8 °C. Consulte el manual del producto para conocer el contenido detallado y las condiciones de almacenamiento para obtener la máxima estabilidad.
Conjugado Biotina
Formato del kit de inmunoensayo Sandwich ELISA Kit
Tipo de producto Kit de ELISA
Tipo de muestra Plasma, Sobrenadante, Suero
Tipo de sustrato Proteases
Especies diana Humano
Para utilizar con (aplicación) Detección colorimétrica
Para utilizar con (equipo) Lector de microplacas colorimétrico
Alias de gen 72 kDa gelatinase; 72 kDa type IV collagenase; 72 kDa type IV collagenase isoform a preproprotein; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; CLG4; CLG4A; Collagenase; collagenase type IV-A; endopeptidase; GelA; gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloprotease 2; matrix metalloproteinase; matrix metalloproteinase 2; matrix metalloproteinase 2 (72 KDa type IV collagenase); matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP2; MMP-2; MMP-2 protein; MMP-II; MMPs; MONA; neutrophil gelatinase; PEX; prepro-72kDa matrix metalloproteinase; Progelatinase A; TBE1; TBE-1; wu:fa99h12; wu:fk89d0172 kDa gelatinase; 72 kDa type IV collagenase; 72 kDa type IV collagenase isoform a preproprotein; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; CLG4; CLG4A; Collagenase; collagenase type IV-A; endopeptidase; GelA; gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloprotease 2; matrix metalloproteinase; matrix metalloproteinase 2; matrix metalloproteinase 2 (72 KDa type IV collagenase); matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP2; MMP-2; MMP-2 protein; MMP-II; MMPs; MONA; neutrophil gelatinase; PEX; prepro-72kDa matrix metalloproteinase; Progelatinase A; TBE1; TBE-1; wu:fa99h12; wu:fk89d01; CLG4; CLG4A; MMP-2; MMP-II; MONA; TBE-1
ID de gen (Entrez) 4313
Símbolo de gen CLG4,CLG4A,MMP-2,MMP-II,MONA,TBE-1
Tiempo de incubación 4 h
CV interensayo 5,6%
CV intraensayo 3,3%
Contenido del kit Placa de 96 pocillos recubiertos previamente, Estándar, Tampón de dilución estándar, Anticuerpo de detección biotinilado, Estreptavidina-HRP, Diluyente HRP, Tampón de lavado, Cromógeno, Solución de parada, Cubiertas adhesivas para placas
Etiqueta o tinte Peroxidasa de rábano picante (HRP)
Gen marcador MMP-2
Alias de proteína 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1
Cantidad 96 pruebas
Estado normativo RUO
Volumen de muestra 50 μl de plasma; 50 μl de suero; 50 μl de sobrenadante
Condiciones de envío Wet Ice
Requisitos de almacenamiento De 2 °C a 8 °C
Objetivo Humano
Tiempo de prueba 1 h 20 min
Tiempo total del ensayo 4 h
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Para uso exclusivo en investigación.No apto para uso en procedimientos diagnósticos.

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