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  5. Invitrogen™ Human IL-6 ELISA Kit 

Invitrogen™ Human IL-6 ELISA Kit

Código de producto. 10484943
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Cantidad:
2 x 96 pruebas
5 x 96 pruebas
96 pruebas
Tamaño de la unidad:
192 tests
96 tests
paquete de 5
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Código de producto. Cantidad unitSize
10484943 2 x 96 pruebas 192 tests
10334403 96 pruebas 96 tests
10571855 5 x 96 pruebas paquete de 5
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Código de producto. 10484943 Proveedor Invitrogen™ N.º de proveedor KHC0062
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Descripción

Includes

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Human Interleukin 6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

Información de órdenes

Condición de envío:Hielo húmedo

TRUSTED_SUSTAINABILITY

Specifications

Número de acceso P05231
Intervalo del ensayo 7,8-2500 pg/ml
Sensibilidad del ensayo <2 pg/mL
Contenido y almacenamiento Placas prerecubiertas, patrón o calibrador, anticuerpo detector, conjugado de HRP, diluyentes, tampón de lavado, sustratos y solución de parada. Almacene el kit a 2-8 °C. Consulte el manual del producto para conocer el contenido detallado y las condiciones de almacenamiento para obtener la máxima estabilidad.
Conjugado Biotina
Formato del kit de inmunoensayo Sandwich ELISA Kit
Tipo de producto Kit de ELISA
Familia de proteínas Cytokines & Receptors
Tipo de muestra Plasma, Sobrenadante, Suero
Especies diana Humano
Para utilizar con (aplicación) Colorimétrico
Para utilizar con (equipo) Lector de microplacas colorimétrico
Alias de gen B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
ID de gen (Entrez) 3569
Símbolo de gen BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
CV interensayo 7,9%
CV intraensayo 6,2%
Contenido del kit Placa de 96 pocillos recubiertos previamente, Estándar, Tampón de dilución estándar, Anticuerpo de detección biotinilado, Estreptavidina-HRP, Diluyente HRP, Tampón de lavado, Cromógeno, Solución de parada, Cubiertas adhesivas para placas
Etiqueta o tinte Peroxidasa de rábano picante (HRP)
Precisión <2 pg/ml
Cantidad 2 x 96 pruebas
Estado normativo RUO
Volumen de muestra Plasma, 100 μl; suero, 100 μl; sobrenadante, 100 μl
Condiciones de envío Wet Ice
Requisitos de almacenamiento De 2 °C a 8 °C
Objetivo Humano
Tiempo de prueba 1 h 20 min
Tiempo total del ensayo 3 h
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Para uso exclusivo en investigación.No apto para uso en procedimientos diagnósticos.

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