Melford Nourseothricin Sulfate

In molecular biology, Nourseothricin Sulfate is often employed as a selectable marker in gene expression studies and genetic engineering experiments. It works by inhibiting protein synthesis in bacterial and eukaryotic cells, leading to cell death.

Researchers use this antibiotic to introduce or maintain plasmids carrying resistance genes encoding Nourseothricin acetyltransferase (NAT) enzyme. The NAT enzyme inactivates Nourseothricin, allowing only the transformed cells to survive and propagate.

Nourseothricin Sulfate is also utilized in cell culture and microbiology. It can be used to selectively kill bacterial or fungal contaminants in cell cultures, thereby ensuring the purity of cell lines.