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STEMCELL Technologies Kit de ELISA para TNF-alfa de ratón
Descripción
- The Mouse Tumor Necrosis Factor-alpha (TNF-α) ELISA Kit is designed for the quantitative detection and measurement of mouse TNF-α in biological fluids such as serum, plasma, and cell culture supernatants
- TNF-α is produced by activated T cells and macrophages in response to inflammation or infection
- It is also produced by B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts
- The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine
- The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody
- Addition of the chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample
Especificaciones
Especificaciones
| Descripción | B Cells; Cardiomyocytes, PSC-Derived; Dendritic Cells; Endothelial Cells; Granulocytes and Subsets; Macrophages; Mouse Embryonic Fibroblasts; Myogenic Stem and Progenitor Cells; NK Cells; T Cells |
| Para utilizar con (aplicación) | ELISA |
| Cantidad | 2 placas |
Título del producto
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