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Descripción
The MicroSEQ™ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use the PCR kit to amplify the bacterial 16S rRNA gene, for subsequent sequencing using the MicroSEQ™ Full Gene 16S rDNA Sequencing Kit. This PCR kit does not include a protocol or a Quick Reference Card.
- Sequence-based measurement ensures you get reproducible results in under 24 hours.
- Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
- Universal domain-level primers eliminate up-front optimization.
- MicroSEQ™ ID Analysis Software ensures accurate species identification.
- Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
- Requires minimal training-similar work flow as the MicroSEQ™ D2 LSU fungal kit and the MicroSeq 500 16S rDNA kit.
The MicroSEQ™ ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.
Especificaciones
Especificaciones
| Contenido y almacenamiento | • 2X PCR Master Mix (sufficient for 20 amplifications), 3 tubes • Positive Control DNA, 1 tube • Negative Control DNA (water), 1 tube • Protocol and Quick Reference Guide After first use, store at 2°C to 8°C in a PCR clean room. |
| Método de detección | Sonda de cebado |
| GC-Rich PCR Performance | Low |
| Técnica | SBT (Sequence-Based Typing) |
| Para utilizar con (equipo) | GeneAmp 9700, Veriti Thermal Cycler |
| Bibliotecas | Customizable, Bacterial Library (> 2000 species) |
| Línea de productos | MicroSEQ |
| Tipo de producto | Full Gene 16S rDNA PCR Kit |
| Cantidad | 1 kit |
| Condiciones de envío | Dry Ice |
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
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