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MCF-7(human breast adenocarcinoma) Whole cell lysate, Non-denatured; Abnova

Produktkod. 16049615
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Cantidad:
200 μg
Förpackningsstorlek:
200 microgramos
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Produktkod. Cantidad Förpackningsstorlek
16049615 200 μg 200 microgramos
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Produktkod. 16049615 Leverantör Abnova Leverantörsnummer L046V2.200ug
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Fecha estimada de envoi: 01-12-2026
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Beskrivning

Non-denatured whole cell lysate of MCF-7(human breast adenocarcinoma)

  • Tissue: Mammary Gland; Breast
  • Host: Human
  • Lysis Buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifikationer

Para utilizar con (aplicación) Immunoprecipitation, Western Blot
Tissue Mammary Gland, Breast
Descripción Whole cell lysate (non-denatured)
Tampón de lisis Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Método de preparación Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Pruebas de control de calidad 12.5% SDS-PAGE Stained with Coomassie Blue.
Cantidad 200 μg
Tampón de almacenamiento In modified RIPA Lysis Buffer.
Especie del huésped Human
Contenido y almacenamiento Almacenar a -80°C. Dividir para evitar repetir la congelación y descongelación.
Produkttitel
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