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  6. Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Código de producto. 30245142
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Cantidad:
100 reacciones
120 μg
1600 μg
2000 μL
Tipo de producto:
Kit Lyo-ready RPA
Proteína Lyo-ready T4 Gene 32
Proteína Lyo-ready T4 UvsX
Proteína Lyo-ready T4 UvsY
Tampón de reacción
Tamaño de la unidad:
100 reacciones
120 microgramos
1600 microgramos
2000 microlitros
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Código de producto. Cantidad Tipo de producto unitSize
30245142 100 reacciones Kit Lyo-ready RPA 100 reacciones
30245335 1600 μg Proteína Lyo-ready T4 Gene 32 1600 microgramos
30245214 120 μg Proteína Lyo-ready T4 UvsX 120 microgramos
30245152 120 μg Proteína Lyo-ready T4 UvsY 120 microgramos
30245027 2000 μL Tampón de reacción 2000 microlitros
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Produktkod. 30245142 Leverantör Invitrogen™ Leverantörsnummer A72127

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212.80 EUR hasta el 2026-07-03
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Beskrivning

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
TRUSTED_SUSTAINABILITY

Specifikationer

Tipo de producto Kit Lyo-ready RPA
Formulario Liquid
Para utilizar con (aplicación) RPA, RT-RPA
Polimerasa Lyo-ready Bst DNA Polymerase
Cantidad 100 reacciones
Categoría de investigación Nucleic Acid Amplification
Contenido y almacenamiento

• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl2, 100 μL
• 10 mM dNTP Mix, 50 μL

Store at -25°C to -15°C.
Temperatura óptima de reacción 42°C
Transcriptasa inversa SuperScript IV Reverse Transcriptase
Condiciones de envío Dry Ice
Método de detección End point, real-time, lateral flow
Tiempo de reacción Up to 20 min.
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