RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of approx. 52 kDa. RNaseOUT inhibits RNase A, RNase B, and RNase C.
Applications cDNA synthesis, RT-PCR, and in vitro transcription and translation
Source Purified by affinity chromatography from E. coli expressing a cloned porcine gene
Performance and quality testing SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR
Unit definition One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate
Unit reaction conditions 100mM Tris-acetate (pH 6.5), 1mM EDTA, 0.2mM cCMP, 2 μg RNase A in 1mL from 0 to 10 min at 25°C
PCR and Real-Time PCR, Reverse Transcription
Shipping Condition: Dry ice
For Research Use Only. Not for use in diagnostic procedures.