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  5. Invitrogen™ Kit de ELISA de IL-8 felina

Invitrogen™ Kit de ELISA de IL-8 felina

Código de producto. 17104849
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Cantidad:
5 x 96 pruebas
96 pruebas
Tamaño de la unidad:
1 set
cada uno
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Código de producto. Cantidad Tamaño de la unidad
17104849 5 x 96 pruebas cada uno
15464883 96 pruebas 1 set
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Código de producto. 17104849 Proveedor Invitrogen™ N.º de proveedor EFCXCL8X5
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    Ahorro 206.85€
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Fecha estimada de envoi: 03-11-2026
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Descripción

ELISA

The Feline Interleukin-8/CXCL8 ELISA quantitates Cat IL-8 in feline serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Cat IL-8. Principle of the method The Feline IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso Q9XSX5
Intervalo del ensayo De 24,58 pg/ml a 6000 pg/ml
Sensibilidad del ensayo 25 pg/mL
Intervalo de calibración De 25 pg/ml a 6000 pg/ml
ensayo ELISA sandwich de fase sólida
Conjugado Biotina
Formato del kit de inmunoensayo Sandwich ELISA Kit
Tipo de producto Kit de ELISA
Familia de proteínas GCPR
Tipo de muestra Plasma, Sobrenadante, Suero
Especificidad Este par de anticuerpos ELISA detecta la IL-8 felina. No se han determinado otras especies
Suficiente para 96 ensayos
Especies diana Feline
Para utilizar con (aplicación) ELISA, ensayos de proteínas, biología de proteínas, biología del cáncer, inmunología, neurociencia, neurología, detección de proteínas, análisis de proteínas
Para utilizar con (equipo) Lector de microplacas colorimétrico
Alias de gen (Ala-IL-8)77; (Ser-IL-8)72; Alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; AMCF-I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; CXCL8; cytokine 8; emoctakin; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; GCP-1; Granulocyte chemotactic protein 1; IL8; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin8; interleukin-8; interleuklin-8 precursor; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil activating peptide 1; neutrophil and T-lymphocyte chemotactic and activating factor; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Permeability factor 1; PF1; Protein 3-10C; RP11-537A6.8; RPF1; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1(Ala-IL-8)77; (Ser-IL-8)72; Alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; AMCF-I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; CXCL8; cytokine 8; emoctakin; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; GCP-1; Granulocyte chemotactic protein 1; IL8; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin8; interleukin-8; interleuklin-8 precursor; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil activating peptide 1; neutrophil and T-lymphocyte chemotactic and activating factor; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Permeability factor 1; PF1; Protein 3-10C; RP11-537A6.8; RPF1; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1; IL8
ID de gen (Entrez) 493836
Símbolo de gen CXCL8
CV interensayo < 12%
CV intraensayo < 10%
Contenido del kit Estándar, SAV-HRP, Anticuerpo de detección biotinilado, Cubiertas adhesivas para placas, Placa de 96 pocillos recubiertos previamente, Cromógeno, Concentrado de diluyente de ensayo, Solución de parada, Tampón de lavado
Etiqueta o tinte Peroxidasa de rábano picante (HRP)
Alias de proteína IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8
Cantidad 5 x 96 pruebas
Estado normativo RUO
Disciplina de investigación Matriz extracelular y adhesión celular
Volumen de muestra 50 μl de plasma; 50 μl de suero; 100 μl de sobrenadante
Condiciones de envío Wet or Dry Ice
Requisitos de almacenamiento De 2 °C a 8 °C
Sinónimo IL8
Objetivo Felina
Tiempo de prueba 1 h 20 min
Tiempo total del ensayo 4 h 45 min
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