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Invitrogen™ Human IL-6 Matched Antibody Pair

Código de producto. 10353493
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Cantidad:
10 x 96 Tests
Tamaño de la unidad:
1 PCS
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10353493 10 x 96 Tests 1 PCS
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Código de producto. 10353493 Proveedor Invitrogen™ N.º de proveedor CHC1263
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Descripción

ELISA

The Human IL-6 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-6. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso P05231
Intervalo del ensayo 15.6-1000 pg/mL
Sensibilidad del ensayo 15.6 pg/mL
Conjugado HRP
N.º de referencia del gen P05231
Alias de gen BSF2, HGF, HSF, IFNB2, IL-6, B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Símbolos de los genes IL6
Formato del kit de inmunoensayo Matched Antibody Pair
Tipo de producto ELISA, ELISA Kit
Cantidad 10 x 96 Tests
Tipo de muestra Plasma, Serum, Supernatant
ID de gen (Entrez) 3569
Especies diana Human
Para utilizar con (equipo) Absorbance Microplate Reader, Colorimetric Microplate Reader
Incluye Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Contenido y almacenamiento Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Símbolo de gen Il6
Contenido del kit Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etiqueta o tinte Biotin
Estado normativo RUO
Condiciones de envío Wet Ice
Requisitos de almacenamiento 2-8°C
Objetivo IL-6
Tiempo de prueba 1 hr 30 min
Tiempo total del ensayo 24 hr
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