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Invitrogen™ Silencer™ Negative Control No. 1 siRNA

Silencer™ Negative Control No. 1 siRNA

Marca:  Invitrogen™ AM4635

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 10793457

  • 674.00€ / cada uno


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Descripción

Descripción

Invitrogen™ Silencer™ Negative Control #1 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology.
  • Nontargeting with limited sequence similarity to known genes
  • Validated for use in human, mouse, and rat cells
  • Functionally proven to have minimal effects on cell proliferation and viability
  • HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the “hit” threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer™ siRNA Screening Control Panel (SKU#AM4640)
The Silencer™ siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer™ siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Invitrogen™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
Especificaciones

Especificaciones

RNAi, transfection
The siRNA is provided dried down in a single tube, along with Nuclease-free Water
40nmol
–20°C
Videos
Documentos

For Research Use Only. Not for use in diagnostic procedures.