Invitrogen™ Human Progesterone Receptor (aa 533-547) Synthetic Peptide

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities. PEP-016 is a 15 amino acid (amino acids 533-547) synthetic peptide whose sequence is found N-terminal to the PR DNA binding domain. The sequence of this peptide is (amino to carboxy terminus): G-L-P- Q-V-Y-P-P-Y-L-N-Y-L-R-PThis peptide may be used for neutralization and control experiments with the monoclonal antibody that reacts with this product and PR, catalog &Num; MA1-410. Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.Reconstitute with 0.1 mL of distilled water.