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Invitrogen™ Human IL-6 Rapid ELISA Kit

Código de producto. 30828487
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96 pruebas
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30828487 96 pruebas
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Código de producto. 30828487 Proveedor Invitrogen™ N.º de proveedor EELR002

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Descripción

ELISA

The Human Interleukin 6 (IL 6) ELISA quantitates IL 6 in serum and plasma. Principle of the method The Human IL 6 solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody. A substrate solution is added that reacts with the enzyme antibody target complex to produce a measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso P05231
Intervalo del ensayo 3.13-200 pg/mL
Sensibilidad del ensayo 1.02 pg/mL
Conjugado HRP
Formato del kit de inmunoensayo Coated ELISA Kit
Tipo de producto ELISA
Tipo de muestra Plasma, Serum
Especies diana Human
Para utilizar con (equipo) Absorbance Microplate Reader
Alias de gen B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6;
ID de gen (Entrez) 3569
Símbolo de gen Il6
CV interensayo <10%
CV intraensayo <10%
Contenido del kit Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Etiqueta o tinte HRP
Cantidad 96 pruebas
Estado normativo RUO
Volumen de muestra Plasma 50 μL, Serum 50 μL
Condiciones de envío Wet or Dry Ice
Requisitos de almacenamiento Refer to product documentation for component specific storage temperature.
Objetivo IL-6
Tiempo de prueba 15 min
Tiempo total del ensayo 1 hr 30 min
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