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Invitrogen™ Human IL-2 ELISA Kits
Measure levels of human interleukin-2 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.
Marca: Invitrogen™ ESS0010
Detalles adicionales : Peso : 0.10000kg
Descripción
Assay IL-2 (human interleukin-2) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-2 ELISA Kits.
These human IL-2 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-2 (IL-2) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-2 antibody pairs and IL-2 standard only. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human interleukin-2 (IL-2)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 38.4 to 1500pg/mL
Sensitivity: < 6pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ, GM-CSF, INFγ, or mouse IL-2
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
Especificaciones
| 38.4 to 1500pg/mL | |
| Human IL-2 ELISA Reagent Kit | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum | |
| 5 x 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 5 Plate Kit | |
| Natural and recombinant human interleukin-2 (IL-2) |
| <6pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with human IL-1a, IL-1ß, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß, GM-CSF, INFg, or mouse IL-2 | |
| TMB Substrate Solution, 55mL, Streptavidin-HRP Conjugate, 0.25mL, Stop Solution, 55mL, IL-2 Detection Antibody, 1 vial, Human IL-2 Standard, 5 vials, IL-2 Coating Antibody, 1 vial | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| 50 μL | |
| 4 hr. |