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Thermo Scientific™ Células GeneBLAzer™ PR-UAS-bla HEK 293T
PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Marca: Thermo Scientific™ K1693
Descripción
PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of R5020. In addition, PR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Especificaciones
GW 3965, 24 (S), 25-epoxicolesterol, 22 (R) OH-colesterol, 24 (S) OH-colesterol | |
Células divisoras | |
Fluorescente | |
Farmacología y biofarmacología, identificación y validación de dianas y secuencias líder | |
2 x 106 | |
GeneBLAzer™ | |
Célula HEK 293T | |
BLA (beta-lactamasa) | |
Células GeneBLAzer™ PR-UAS-bla HEK 293T |
Gen informador de beta lactamasa a base de células | |
Envío: Hielo seco Almacenamiento: -80 °C/Nitrógeno líquido Contiene: Células PR-UAS-bla HEK 293T (K1103) | |
Células GeneBLAzer™ PR-UAS-bla células HEK 293T, 2 x 106 células, líquido, línea de células HEK 293T, especie humana, estado de células con división, método de detección fluorescente, objetivo de receptores nucleares, indicador BLA (beta-lactamasa), fenotipo de células epiteliales, propiedades de crecimiento adherentes | |
Receptores nucleares | |
TO901317 | |
2 x 106 células | |
293T (HEK) | |
PGR, NR3C3, PR |
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.