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Thermo Scientific™ Fluorescein-D22 Coactivator Peptide, 100 μM

Código de producto. 10036842
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Cantidad:
100 μl
1 ml
Tamaño de la unidad:
1 ml
cada uno
Código de producto. Cantidad unitSize
10036842 1 ml 1 ml
10556203 100 μl cada uno
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Code produit. 10036842

Marque: Thermo Scientific™ PV4387

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LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.

LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work

Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

TRUSTED_SUSTAINABILITY

Spécification

Pureza ≥95% by HPLC
Nombre común Fluorescein - D22 Coactivator Peptide
Concentración 100 μm
Contenido y almacenamiento Almacene los péptidos a -20 °C.
Descripción Fluorescein-D22 Coactivator Peptide, 1mL, 100μM Concentration, 2499Da Molecular Weight, –5 to –30°C Storage Condition, Used for Pharma and Biopharma, Target and Lead Identification and Validation
Para utilizar con (aplicación) Pharma and biopharma, target and lead identification and validation
Tipo de etiqueta Colorantes clásicos
Peso molecular 2499Da
Tipo de producto Fluorescein - D22 Coactivator Peptide
Cantidad 1 ml
Estado normativo For research use only. Not for use in diagnostic procedures
Secuencia Fluorescein- LPYEGSLLLKLLRAPVEEV
Condiciones de envío Hielo seco
Tampón de almacenamiento 10mM Na2CO3 (pH 11.0)
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

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