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Thermo Scientific™ SP6 RNA Polymerase, HC (≥100 U/µL)

Thermo Scientific™ SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Marca:  Thermo Scientific™ EP0133

Detalles adicionales : Peso : 0.00900kg

Código de producto. 10899720

  • 170.00€ / 5000 unidades


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Descripción

Descripción

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

Especificaciones

Especificaciones

1000U
SP6 RNA Polymerase
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