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Maxima Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Marca: Thermo Scientific™ K0242
Detalles adicionales : Peso : 0.08000kg
Descripción
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.
- Especificidad: la ADN polimerasa Taq Maxima Hot Start y el tampón optimizado eliminan la formación y amplificación inespecífica de los dímeros de primers.
- Sensibilidad: detecta un número bajo de copias.
- Amplia gama lineal: cuantificación precisa en 9 órdenes de magnitud.
- Reproducibilidad y comodidad: mezcla maestra 2X lista para su uso.
Especificaciones
| Contains: • 10 x 1.25 mL Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with fluorescein passive reference dye; sufficient for 1000 x 25 μL reactions. • 10 x 1.25 mL Nuclease-Free Water Store at -20°C. |
|
| Tubo | |
| qPCR | |
| Estándar | |
| Maxima | |
| 1000 Reacciones a 25 μl | |
| 2X | |
| ADN, ADNbc |
| SYBR | |
| Alto | |
| ADN polimerasa Taq | |
| BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5 | |
| Mezcla maestra de PCR en tiempo real SYBR | |
| PCR en tiempo real (PCRq) | |
| 1.000 reacciones | |
| 10 x 1,25 ml |
For Research Use Only. Not for use in diagnostic procedures.