Invitrogen™ F4/80 Monoclonal Antibody (BM8), Brilliant Ultra Violet™ 395, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

The BM8 MAb reacts with mouse F4/80 antigen, an app. 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80. Applications Reported: This BM8 Ab has been reported for use in flow cytometric analysis. Applications Tested: This BM8 Ab has been tested by flow cytometric analysis of mouse resident peritoneal macrophages. This may be used at less than or equal to 1ug per test. A test is defined as the amount (ug) of Ab that will stain a cell sample in a final volume of 100uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. BUV395 is a dye that emits at 395nm and is intended for use on cytometers equipped with an UV (355nm) laser. Excitation: 350nm; Emission: 395nm; Laser: UV Laser.