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Invitrogen™ F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 488

Código de producto. 31722864
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Cantidad:
250 μL
Tamaño de la unidad:
250 microlitros
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31722864 250 μL 250 microlitros
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Código de producto. 31722864 Proveedor Invitrogen™ N.º de proveedor SA510382AFP488

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Descripción

F(ab)-Goat Recombinant Monoclonal Secondary Antibody

F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 is designed to specifically detect the mouse IgG2b isotype showing no detectable reactivity with immunoglobulins from any other tested species. This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno Mouse IgG2b
Aplicaciones Immunohistochemistry (Paraffin), Immunocytochemistry
Clasificación Recombinant Monoclonal
Clon 27H27L7
Concentración 1 mg/mL
Conjugado Alexa Fluor Plus 488
Formulación Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
Alias de gen IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Especie del huésped Goat
Inmunógeno Purified Mouse IgG.
Método de purificación Affinity chromatography
Cantidad 250 μL
Estado normativo RUO
Primario o secundario Secondary
Especies diana Mouse
Contenido y almacenamiento 4°C, store in dark, DO NOT FREEZE!
Tipo de producto Secondary Antibody
Formulario Liquid
Isotype IgG F(ab)
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